Santa Cruz TGFα Antibody (D-6)

Transforming growth factor-a (TGFa) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFa is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFa binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-b. TGFa is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells.
TGFa bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
TGFa (D-6) is available conjugated to agarose (sc-374433 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-374433 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; to either phycoerythrin (sc-374433 PE), fluorescein (sc-374433 FITC), Alexa Fluor 488 (sc-374433 AF488), Alexa Fluor 546 (sc-374433 AF546), Alexa Fluor 594 (sc-374433 AF594) or Alexa Fluor 647 (sc-374433 AF647),
200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Alexa Fluor 680 (sc-374433 AF680) or Alexa Fluor 790 (sc-374433 AF790), 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM.
Blocking peptide available for competition studies, sc-374433 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizer protein).