5,5'-dithio-bis-(2-nitrobenzoic acid) (DTNB)
Ellman's reagent is a versatile, water-soluble compound for quantifying free sulfhydryl groups in solution. It reacts with a free sulfhydryl group to yield a mixed disulfide and 2-nitro-5-thiobenzoic acid (NTB), a measurable yellow colored product at 412nm.
Ellman's reagent is very useful as a free sulfhydryl assay reagent due to its high specificity for -SH groups at neutral pH, high molar extinction coefficient and short reaction time.
Synonyms: DTNB; 5,5'-Dithio-bis(2-nitrobenzoic acid); Ellman’s Reagent; 3-Carboxy-4-nitrophenyl disulfide
Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.
G-Bioscience Ellman’s Reagent
#Grup produk P118001029
Spesifikasi
| Cat. No. | BC87 |
|---|---|
| Citations | 8 |
| PubChem Chemical ID | 6254 |
| Size (g) | 5 |
| Storage Temperature (°C) | 2 to 8 |
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Description
Feature
- Highly stable and water soluble- Quick and simple assay
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