CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+ cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+ cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.
Application Notes
Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4 Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.
Biolegend Anti-CD90 Antibody
#Grup produk P118000224
Spesifikasi
| Antibody Type | Monoclonal |
|---|---|
| Application | FC - Quality tested |
| Clone | 5E10 |
| Concentration | Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) |
| Excitation Laser | Blue Laser (488 nm); Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA) |
| Host Species | Mouse |
| Immunogen | HEL cells |
| Isotype | Mouse IgG1, κ |
| Isotype Control | PE Mouse IgG1, κ Isotype Ctrl (FC) |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions |
| Regulatory Status | RUO |
| Reported Reactivity | African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Pig |
| Size (tests) | 100 |
| Storage and Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze. |
| Workshop | HCDM listed |
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Informasi Produk
Description
Use
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.Produk Terpopuler
20%
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