Application Notes
Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).
Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.
Biolegend Anti-CD44 Antibody
#Grup produk P118000163
Spesifikasi
| Antibody Type | Monoclonal |
|---|---|
| Application | FC - Quality tested |
| Excitation Laser | Blue Laser (488 nm); Green Laser (532 nm)/Yellow-Green Laser (561 nm) |
| Host Species | Rat |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells |
| Isotype Control | PE Rat IgG2b, κ Isotype Ctrl |
| Preparation | The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. |
| Size (tests) | 100 |
| Storage and Handling | The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze |
| Verified Reactivity | Mouse, Human |
| µg Sizes | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide |
| µg Sizes (mg/ml) | 0.2 |
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Informasi Produk
Description
Use
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For µg sizes, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.Produk Terpopuler
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