Sigma-Aldrich HypoThermosol FRS Preservation Solution (Hypothermic Preservation Medium)

Application
HypoThermosol is an optimized hypothermic (2-8°C) preservation media that enables improved and extended preservation of cells, tissues and organs. HypoThermosol-FRS is uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process.
HypoThermosol-FRS includes key ions at concentrations that balance the intracellular state at hypothermic temperatures. Additional components include pH buffers, energy substrates, free radical scavengers, and osmotic/oncotic stabilizers.
Preparation Note
Usage and Preservation Protocol
Wipe down outside of container with 70% ethanol before opening.Contents are sterile. If seal has been broken, do not use and contact BioLife Solutions.HypoThermosol FRS is ready to use. Simply replace culture medium with chilled (<10°C) HypoThermosol FRS and maintain at 2-8°C.At the end of the cold storage period, simply remove samples from the cold, decant the cold HypoThermosol FRS and replace with warm (20-37°C) culture medium of choice.
HypoThermosol FRS is not designed as a cell culture medium and should not be used above 15°C or warmed to 37°C