RNase A is an endoribonuclease that is from bovine pancreas for molecular biology applications. The major application for RNase A is the removal of RNA from preparation of plasmid DNA as well as extraction of plasmid DNA. It is also used in removal of unspecifically bound RNA; RNase protection assays; analysis of RNA sequences as well as hydrolysis of RNA contained in protein samples.
RNase A attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.
Vivantis R Nase A >2500 U/mg250 M G
#Grup produk P106500518
Spesifikasi
| Size (mg) | 250 |
|---|---|
| Grade | Molecular Biology |
| Appearance | White lyophilized powder; a chromatographically purified, lyophilized powder. |
| Activity (Kunitz) | ≥60 units/mg protein |
| CAS RN | 9001-99-4 |
| Source | Bovine pancreasAll products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for e |
| Molecular mass | 13.7 kDa (amino acid sequence) |
| Extinction coefficient | E1% = 7.1% (280nm) |
| Inhibitors | Ribonuclease inhibitor |
| Isoelectric point | pI: 9.6 |
| Optimum pH | 7.5 (activity range of 6 - 10) |
| Optimum temperature | 60°C (activity range of 15 - 70°C) |
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