Merck Millipore ALDH Detection Assay

The AldeGreen ALDH Detection Assay enables flow cytometry-based detection of intracellular Aldehyde Dehydrogenase (ALDH) enzyme activity. High ALDH activity serves as a universal marker of stem cells, both normal and malignant. The assay employs a fluorescent and non-toxic ALDH substrate (AldeGreen) that diffuses freely into intact and viable cells, but remains trapped inside the cells once converted by ALDH into the corresponding acid. The amount of fluorescence produced is proportional to the ALDH activity in the cells and is measured by flow cytometry, allowing fluorescence-activated cell sorting (FACS). This kit supplies the ALDH inhibitor diethylaminobenzaldehyde (DEAB), which is used in negative control testing necessary for background fluorescence assessment.