Merck Millipore CpGenome Universal Unmethylated DNA Set S7822 1pc

Methylation of cytosines located 5' to guanosine is known to have a profound effect on the expression ofany eukaryotic genes. In noal cellsethylation occurs predinantly in Cpoor regions, while Crich areas, called Cpislands rain uethylated. The exceptions are the extensiveethylation of CpG islands associated with transcriptional inactivation of regulatory regions of printed genes and genes on the inactive chrose of fales. Aberrantethylation of noally uethylated CpG islands has been docented as a relatively frequent event in mortalized and transfoed cells and has been associated with transcriptional inactivation of defined tor suppresser genes in han cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypeethylation in tors.Severalethods have been developed to deteine theethylation status of cytosine. These include digestion withethylation sensitive restriction enzes as in restriction lanark genic scanning, oligonucleotide arrays, genic DNA sequencing andethylation specific PCR SP). Se techniques areore useful for discovery while others are better used foronitoring of knownethylated cytosines. Genic DNA sequencing, although te consing and labor intensive, offers aore universal detectionethod.SP is now an established technology for theonitoring of abnoal geneethylation in selected gene sequences. Utilizing all ounts of DNA, this procedure offers sensitive and specific detection ofethylcytosine in proters. It is being exploited to define tor suppresser gene function, and to provide a new strategy for early tor detection.The initial step of both genic sequencing andSP is to perfo a bisulfiteodification of the DNA sple.SP then involves PCR plification with specific prers designed to distinguishethylated fr uethylated DNA.